Since the contact frequency between loci pairs strongly correlates with separation on the genome, Hi-C has rapidly gained popularity as an economical method for generating chromosome-scale scaffolds ( Burton et al., 2013 Dudchenko et al., 2017). Hi-C is a sequencing-based proximity ligation assay that provides contact information between pairs of loci, originally designed to study the 3D structure of the genome inside a cell nucleus ( Lieberman-Aiden et al., 2009). Long-distance linkage information, such as physical maps, genetic maps, optical maps and Hi-C contact maps, is often used to construct chromosome-scale scaffolds from contigs. Despite the technological advances, the assembly of reference-quality genomes with long-read sequencing data alone remains elusive ( Amarasinghe et al., 2020). Multiple genome sequencing projects have been launched in the past few years, such as the Earth Biogenome Project ( Lewin et al., 2018), the Vertebrate Genomes Project ( Rhie et al., 2021) and the Darwin Tree of Life Project (DToL, Blaxter et al., 2022) aiming to assemble high quality, chromosome-scale genomes for many thousands of species across a range of genome sizes, complexity and ploidy. The rapid revolution of long-read, single-molecule DNA sequencing technologies in read length, base accuracy and per-base cost is driving a golden age for de novo genome assembly.
0 Comments
Leave a Reply. |
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |